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Table of Contents

NanoCuvette™ One

Basics

Flyer

The NanoCuvette™ One expands the capabilities of an UV-VIS spectrophotometer to include quantification via refractive index for QC, kinetics and 0.5 µL sample measurements.

Product Description

The NanoCuvette™ One expands the capabilities of a UV-Vis spectrophotometer to include quantification of non absorbing compounds via refractive index. Use cases include label-free kinetics, label-free concentration determinations, and the possibility to measure on 0.5 μL droplets for low-volume samples. In addition, a free cloud-based online software is available as a lab journal system to analyse spectra, automatically extract results and save information.

Cleaning Manual

It is strongly recommended that each cuvette is only used once, as the product cannot be guaranteed to perform nominally after sample contact. However, the following cleaning protocol has been tested for reusability of the product when used with sugary samples (e.g. undiluted honey).This cleaning manual is provided “as-is” and no warranties are given.

Data sheet

Data sheet for NanoCuvette™ One.

Product Brief

The NanoCuvette™ One is an excellent improvement in workflow for quantitative analysis in numerous industries such as biotechnology, pharmaceuticals, chemistry, foods and industrial applications for quality control.

Product Brief (Danish)

NanoCuvette ™ One er en fremragende forbedring af arbejdsprocessen ved kvantitativ analyse indenfor adskillige fag såsom bioteknologi, lægemidler, kemi, fødevarer og industriel brug til kvalitetskontrol.

Press release (Danish)

Copenhagen Nanosystems lancerer verdens første plastikbeholder med nanoteknologi. I stedet for dyre instrumenter kan væsker nu analyseres på sekunder med billig og genbrugelig plastikbeholder.

Application notes

Droplets and low volume samples

Low volume samples around 0.5 µL can be measured at low cost
in a conventional spectrophotometer by dispensing a droplet and flattening it on the optical filter of NanoCuvette™ One. This method enables measurements of relevant static or kinetic parameters at low sample volumes. DI water and sucrose samples are used to demonstrate the method.

Enzymes and kinetics

Hexokinase activity can be easily detected label-free in real-time with NanoCuvette™ One.

Protein concentrations and Refractometry

Protein concentration determination is fast and easy with the novel NanoCuvette™ One.

Saliva and starch degradation

NanoCuvette™ One can be used to study the degradation of starch over time by amylase enzymes from saliva.

Glycerol concentration and QC

Quality control of glycerol in a solution can be done fast and easy with the novel NanoCuvette™ One.

Buffers and QC

NanoCuvette™ One is used to quickly determine the quality of a buffer.

Sugar and Refractometry

Refractive measures are widely used in the sugar industry for rapid quantification of the sugar content in sugar solutions.

Honey and Water Content

NanoCuvette™ One offers a quick and easy way to investigate honey quality and moisture content.

Coffee and Sugar

NanoCuvette™ One offers a quick and easy way of detecting sugar in your coffee.

Apple juice and QC

Refractometry is widely used in the quality control of juice and other beverages.

Technical notes

Protein calculations

The concentration of proteins in solution can be measured by refractive index. In addition to the functionality in SpectroWorks™, ready-to-use Excel spreadsheets for protein concentration from refractive index are provided. These spreadsheets are designed to work with data from NanoCuvette™ One. Thereby, proteins can be quantified label-free using a spectrophotometer without UV absorbance or fluorescence at volumes 0.5 µL or 2 mL.

 

Protein Concentration 1 – Spreadsheet (xlsx)

 

Teaching material

Fortyndingsrække med honning – elev (Danish)

Med denne øvelsesvejledning vil du lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i honning.

Fortyndingsrække med honning – lærer (Danish)

Med denne øvelsesvejledning vil eleverne lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i honning, ved at lave fortyndingsserie

Forskel på honning – elev (Danish)

Med denne øvelsesvejledning vil du lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i forskellige honninger.

Forskel på honning – lærer (Danish)

Med denne øvelsesvejledning vil eleverne lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i forskellige honninger, ved at måle på forskellige typer af honning.

Ukendt honning – elev (Danish)

Med denne øvelsesvejledning vil du lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i ukendte honninger.

Ukendt honning – lærer (Danish)

Med denne øvelsesvejledning vil eleverne lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i forskellige honninger, ved at måle på kendte og ukendte honninger.

SpectroWorks™

Basics

User guide

Steps for using SpectroWorks™ with NanoCuvette™ One.

SpectroLink™

Basics

Flyer

SpectroLink™ connects to UV-VIS spectrophotometers to aquire, analyze and extract results in one easy workflow via the online platform SpectroWorks™.

SpectroShell™

Basics

Flyer

SpectroShell™ is an exoskeleton designed for Ocean Insight spectrophotometers that improves repeatability and precision without upgrading instrumentation.

Literature

The core nanotechnology is well described in literature and cphnano is a spin-out from Professor A. Kristensens group at the Department of Nanotechnology, Technical University of Denmark. Peer-reviewed publications on the underlying technology and applications can be found here:
FigureTitle CitationDOI LinkPDF
Label-free monitoring of diffusion in microfluidicsSørensen, K. T., & Kristensen, A. (2017). Micromachines, 8(11), [329].https://doi.org/10.3390/mi8110329DTU Orbit
All-polymer photonic crystal slab sensorHermannsson, P. G., Sørensen, K. T., Vannahme, C., Smith, C., Klein, J. J., Russew, M-M., … Kristensen, A. (2015). Optics Express, 23(13), 16529-16539.https://doi.org/10.1364/OE.23.016529DTU Orbit
High frame rate multi-resonance imaging refractometry with distributed feedback dye laser sensorVannahme, C., Dufva, M., & Kristensen, A. (2015), Light: Science & Applications, 4(4), [e269].https://doi.org/10.1038/lsa.2015.42DTU Orbit
Refractive index dispersion sensing using an array of photonic crystal resonant reflectorsHermannsson, P. G., Vannahme, C., Smith, C., Sørensen, K. T., & Kristensen, A. (2015). Applied Physics Letters, 107(6), [061101].https://doi.org/10.1063/1.4928548DTU Orbit
Refractometric monitoring of dissolution and fluid flow with distributed feedback dye laser sensorVannahme, C., Sørensen, K. T., Gade, C., Dufva, M., & Kristensen, A. (2015). Optics Express, 23(5), 6562-6568.https://doi.org/10.1364/OE.23.006562DTU Orbit
-Absolute analytical prediction of photonic crystal guided mode resonance wavelengthsP. G. Hermannsson, C. Vannahme, C. L. C. Smith, & A. Kristensen. Appl. Phys. Lett. 105, 071103 (2014).https://doi.org/10.1063/1.4893664-
-Accurate wavelength prediction of photonic crystal resonant reflection and applications in refractive index measurementP. G. Hermannsson,
C. Vannahme, C. L. C. Smith & A. Kristensen. Sensors IEEE, (2014).
https://doi.org/10.1109/ICSENS.2014.6985274-