Overview
Documentation
This page provides a list of different documents and material for reference.
Table of Contents
NanoCuvette™ One
Basics
Flyer
The NanoCuvette™ One expands the capabilities of an UV-VIS spectrophotometer to include quantification via refractive index for QC, kinetics and 0.5 µL sample measurements.
Product Description
The NanoCuvette™ One expands the capabilities of a UV-Vis spectrophotometer to include quantification of non absorbing compounds via refractive index. Use cases include label-free kinetics, label-free concentration determinations, and the possibility to measure on 0.5 μL droplets for low-volume samples. In addition, a free cloud-based online software is available as a lab journal system to analyse spectra, automatically extract results and save information.
Cleaning Manual
It is strongly recommended that each cuvette is only used once, as the product cannot be guaranteed to perform nominally after sample contact. However, the following cleaning protocol has been tested for reusability of the product when used with sugary samples (e.g. undiluted honey).This cleaning manual is provided “as-is” and no warranties are given.
Data sheet
Data sheet for NanoCuvette™ One.
One pager
The NanoCuvette™ One is a new Danish product based on state-of-the-art nanotechnology that transform a popular laboratory instrument into a one-size-fit-all solution for education, laboratories and production facilities. Together with the free online software, the NanoCuvette™ One provides a turnkey solution in a standard laboratory format without any up-front investment. Just put your sample in the NanoCuvette™ One and you are ready to go!
Press release (Danish)
Copenhagen Nanosystems lancerer verdens første plastikbeholder med nanoteknologi. I stedet for dyre instrumenter kan væsker nu
analyseres på sekunder med billig og genbrugelig plastikbeholder.
Application notes
Droplets and low volume samples
Low volume samples around 0.5 µL can be measured at low cost
in a conventional spectrophotometer by dispensing a droplet and flattening it on the optical filter of NanoCuvette™ One. This method enables measurements of relevant static or kinetic parameters at low sample volumes. DI water and sucrose samples are used to demonstrate the method.
Enzymes and kinetics
Hexokinase activity can be easily detected label-free in real-time with NanoCuvette™ One.
Protein concentrations and Refractometry
Protein concentration determination is fast and easy with the novel NanoCuvette™ One.
Saliva and starch degradation
NanoCuvette™ One can be used to study the degradation of starch
over time by amylase enzymes from saliva.
Glycerol concentration and QC
Quality control of glycerol in a solution can be done fast and easy with the novel NanoCuvette™ One.
Buffers and QC
NanoCuvette™ One is used to quickly determine the quality of a buffer.
Sugar and Refractometry
Refractive measures are widely used in the sugar industry for rapid quantification of the sugar content in sugar solutions.
Honey and Water Content
NanoCuvette™ One offers a quick and easy way to investigate honey quality and moisture content.
Coffee and Sugar
NanoCuvette™ One offers a quick and easy way of detecting sugar in your coffee.
Apple juice and QC
Refractometry is widely used in the quality control of juice and other beverages.
Technical notes
Protein calculations
The concentration of proteins in solution can be measured by refractive index. In addition to the functionality in SpectroWorks™, ready-to-use Excel spreadsheets for protein concentration from refractive index are provided. These spreadsheets are designed to work with data from NanoCuvette™ One. Thereby, proteins can be quantified label-free using a spectrophotometer without UV absorbance or fluorescence at volumes 0.5 µL or 2 mL.
Protein Concentration 1 – Spreadsheet (xlsx)
Teaching material
Fortyndingsrække med honning – elev (Danish)
Med denne øvelsesvejledning vil du lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i honning.
Fortyndingsrække med honning – lærer (Danish)
Med denne øvelsesvejledning vil eleverne lære at forstå hvordan
brydningsindekset ændres i overensstemmelse med vandindholdet i honning, ved at lave fortyndingsserie
Forskel på honning – elev (Danish)
Med denne øvelsesvejledning vil du lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i forskellige honninger.
Forskel på honning – lærer (Danish)
Med denne øvelsesvejledning vil eleverne lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i forskellige honninger, ved at måle på forskellige typer af honning.
Ukendt honning – elev (Danish)
Med denne øvelsesvejledning vil du lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i ukendte honninger.
Ukendt honning – lærer (Danish)
Med denne øvelsesvejledning vil eleverne lære at forstå hvordan brydningsindekset ændres i overensstemmelse med vandindholdet i forskellige honninger, ved at måle på kendte og ukendte honninger.
SpectroWorks™
Basics
User guide
Steps for using SpectroWorks™ with NanoCuvette™ One.
SpectroLink™
Basics
Flyer
SpectroLink™ connects to UV-VIS spectrophotometers to aquire, analyze and extract results in one easy workflow via the online platform SpectroWorks™.
SpectroShell™
Basics
Flyer
SpectroShell™ is an exoskeleton designed for Ocean Insight spectrophotometers that improves repeatability and precision without upgrading instrumentation.
Literature
The core nanotechnology is well described in literature and cphnano is a spin-out from Professor A. Kristensens group at the Department of Nanotechnology, Technical University of Denmark. Peer-reviewed publications on the underlying technology and applications can be found here:| Figure | Title | Citation | DOI Link | |
|---|---|---|---|---|
 | Label-free monitoring of diffusion in microfluidics | Sørensen, K. T., & Kristensen, A. (2017). Micromachines, 8(11), [329]. | https://doi.org/10.3390/mi8110329 | DTU Orbit |
 | All-polymer photonic crystal slab sensor | Hermannsson, P. G., Sørensen, K. T., Vannahme, C., Smith, C., Klein, J. J., Russew, M-M., … Kristensen, A. (2015). Optics Express, 23(13), 16529-16539. | https://doi.org/10.1364/OE.23.016529 | DTU Orbit |
 | High frame rate multi-resonance imaging refractometry with distributed feedback dye laser sensor | Vannahme, C., Dufva, M., & Kristensen, A. (2015), Light: Science & Applications, 4(4), [e269]. | https://doi.org/10.1038/lsa.2015.42 | DTU Orbit |
 | Refractive index dispersion sensing using an array of photonic crystal resonant reflectors | Hermannsson, P. G., Vannahme, C., Smith, C., Sørensen, K. T., & Kristensen, A. (2015). Applied Physics Letters, 107(6), [061101]. | https://doi.org/10.1063/1.4928548 | DTU Orbit |
 | Refractometric monitoring of dissolution and fluid flow with distributed feedback dye laser sensor | Vannahme, C., Sørensen, K. T., Gade, C., Dufva, M., & Kristensen, A. (2015). Optics Express, 23(5), 6562-6568. | https://doi.org/10.1364/OE.23.006562 | DTU Orbit |
| - | Absolute analytical prediction of photonic crystal guided mode resonance wavelengths | P. G. Hermannsson, C. Vannahme, C. L. C. Smith, & A. Kristensen. Appl. Phys. Lett. 105, 071103 (2014). | https://doi.org/10.1063/1.4893664 | - |
| - | Accurate wavelength prediction of photonic crystal resonant reflection and applications in refractive index measurement | P. G. Hermannsson, C. Vannahme, C. L. C. Smith & A. Kristensen. Sensors IEEE, (2014). | https://doi.org/10.1109/ICSENS.2014.6985274 | - |