Enzymes 0.5 µL / 2 mL with
Do you want to quantify enzymes label-free?
Introducing label-free kinetics measurements
Direct label-free detection of enzymatic activity with a substrate can be monitored with the change in the intrinsic property, refractive index of the solution as product is formed over time. Traditionally a refractometer has been required for the determination of refractive index. The novel NanoCuvette™ One has a built-in optical filter allowing to measure refractive index change due to enzymatic reaction in real time using a conventional spectrophotometer.
All that is needed is our NanoCuvette™ One, 0.5 µL sample and an UV-VIS spectrophotometer for instance Shimadzu, PerkinElmer, Thermo Ficher, Mettler Toledo, VWR or similar.