---
title: NanoCuvette™ One - cphnano.com
description: Affordable upgrade for your existing UV-Vis spectrophotometer. The NanoCuvette™ is an excellent improvement in workflow for quantitative analysis.
image: https://cphnano.com/hubfs/PHOS_20181221_nanocuvette_being_held_small.png
---

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# NanoCuvette™ One

 

#### Enter the Future of Lab Work

## Next-gen Quantitative Analysis

The [NanoCuvette™ One](https://knowledge.cphnano.com/en/pages/nanocuvette-one-product-description) is an excellent improvement in any laboratory workflow for [quantitative analysis](https://knowledge.cphnano.com/en/pages/uv-vis-common-uses) in numerous industries such as biotechnology, pharmaceuticals, chemistry, foods and industrial applications for quality control.

The capacity of NanoCuvette™ One goes far beyond any product currently on the market in terms of concentration determination. With the NanoCuvette™ One you are able to analyze [smaller samples](https://knowledge.cphnano.com/en/pages/droplet-and-micro-volume-sample-applications-for-nanocuvette-one) (down to 0.5 μL) and a large range of concentrations (0.02 - 300 mg/mL) of non-absorbing compounds.

 

[Product Brief](https://knowledge.cphnano.com/en/pages/nanocuvette-one-product-brief)

[Application Notes](https://knowledge.cphnano.com/en/pages/nanocuvette-one#application-notes)

[![INDS_20201016_Nanocuvette_One_Product_brief_Figure_01_high_res_resized_2240x1794](https://cphnano.com/hubfs/INDS_20201016_Nanocuvette_One_Product_brief_Figure_01_high_res_resized_2240x1794.png "INDS_20201016_Nanocuvette_One_Product_brief_Figure_01_high_res_resized_2240x1794")](https://knowledge.cphnano.com/en/pages/nanocuvette-one-data-sheet)

## Expanding Capabilities

The NanoCuvette™ One expands the capabilities of your spectrophotometer. It allows you to perform classic absorbance (attenuance) spectroscopy and label-free spectroscopy via refractive index. Integrated in the surface is a patented photonic nanocrystal (optical filter), which bends the light beam such that it senses refractive index near the photonic crystal surface in the wavelength range from 550 nm to 800 nm.

## Why Upgrade 

Traditionally, spectrophotometry laboratory work has been limited by lack of robust methods to determine concentrations. We upgrade UV-Vis spectrophotometry around the world and across all major instrument brands to create new ways to determine concentration, perform [enzymatic studies](https://knowledge.cphnano.com/en/pages/enzyme/kinetics) in a label-free, fast, cheap, and reliable method.

## Technology: How We Do It

### Absorption versus label-free spectroscopy

With absorption spectroscopy, the [Beer–Lambert law](https://knowledge.cphnano.com/en/pages/what-is-the-beer-lambert-law) relates the attenuation of light to the properties of the material through which the light is traveling:

A = εcl + A0 ,

![ILLU_20160313_NanoCuvette_Principle_Simple_Abs_275x150](https://cphnano.com/hubfs/ILLU_20160313_NanoCuvette_Principle_Simple_Abs_275x150.png "ILLU_20160313_NanoCuvette_Principle_Simple_Abs_275x150")

where A is the absorbance, ε is molar extinction coefficient, c is the concentration, l is the path length and A0 is the background absorbance.

Lambert, J.H. (1760). Photometria sive de mensura et gradibus luminis, colorum et umbrae \[Photometry, or, On the measure and gradations of light intensity, colors, and shade\] (in Latin). Augsburg, (Germany): Eberhardt Klett.

Beer (1852). "Bestimmung der Absorption des rothen Lichts in farbigen Flüssigkeiten" \[Determination of the absorption of red light in colored liquids\]. Annalen der Physik und Chemie (in German). 162 (5): 78–88. Bibcode:1852AnP...162...78B. doi:10.1002/andp.1852162050

In contrast, when light hits a nanoscale photonic crystal, the resonance wavelength is related to the refractive index or concentration close to the surface:

λ = β(αc + ns) + λ0 ,

![ILLU_20160313_NanoCuvette_Principle_Simple_275x150](https://cphnano.com/hubfs/ILLU_20160313_NanoCuvette_Principle_Simple_275x150.png "ILLU_20160313_NanoCuvette_Principle_Simple_275x150")

where λ is the wavelength, β is a coefficient, α is the specific refractive increment, c is the concentration, ns is the solvent refractive index and λ0 is the reference resonance wavelength, which is derived from [Snell's law](https://en.wikipedia.org/wiki/Snell%27s_law) and this equation is called Hand's Law. In this application this is called [label-free spectroscopy](https://en.wikipedia.org/wiki/Label-free_quantification).

Nano Lett. 2014, 14, 8, 4499–4504Publication Date:July 8, 2014https://doi.org/10.1021/nl5014986Copyright © 2014 American Chemical Society.

Højlund-Nielsen, E., Clausen, J., Mäkela, T., Thamdrup, L. H., Zalkovskij, M., Nielsen, T., Pira, N. L., Ahopelto, J., Mortensen, N. A., Kristensen, A. (2016). Plasmonic Colors: Toward Mass Production of Metasurfaces. Adv. Mater. Technol., 1: 1600054. doi: 10.1002/admt.201600054

## Key Uses

![BLEN_20170901_nanocuvette_one_20191029_transparent_no_reflection_resized_800x1139](https://cphnano.com/hubfs/BLEN_20170901_nanocuvette_one_20191029_transparent_no_reflection_resized_800x1139.png "BLEN_20170901_nanocuvette_one_20191029_transparent_no_reflection_resized_800x1139")

### Lift the Standard of Lab Analysis

- Measurements at different volumes (0.5 μL - 3 mL) and concentrations (0.02 - 300 mg/mL)
- Non-absorbing protein concentrations
- Monitoring label-free enzymatic reactions
- Monitoring non-absorbing/non-fluorescent kinetics
- QC measurements

# Want NanoCuvette™ One or a Demo? 

[Book Online meeting](https://nanocuvette.cphnano.com/meetings/emiho)

![PHOT_20190614_One_droplet_02_white_background_crop_2560px_resized_2100x1126](https://cphnano.com/hubfs/PHOT_20190614_One_droplet_02_white_background_crop_2560px_resized_2100x1126.jpg "PHOT_20190614_One_droplet_02_white_background_crop_2560px_resized_2100x1126")

### Less Sample Needed for Analysis

Enzymes and proteins can be expensive, some equipment needs 2 - 3 μL to measure protein. The NanoCuvette™ One can measure volumes down to 0.5 μL and concentrations down to 0.2 mg/mL for sucrose (Limit of detection is below 0.2 mg/mL sucrose concentration).

## Label-Free Everything

### Micro-Volume Protein Concentration Determination

NanoCuvette™ One’s optical filter measure refractive index and together with SpectroWorks™ it will calculate the refractive index similar to SPR. Refractive index does not require any coloration (dye or indicator) to be measured. Thus it is possible to measure concentrations with spectrophotometer of a transparent solution.

#### Application example: Micro-volume 0.5 uL BSA triplicate measurements

![PYTH_20210625_BSA_conc_Droplet_conc](https://cphnano.com/hubfs/PYTH_20210625_BSA_conc_Droplet_conc.svg "PYTH_20210625_BSA_conc_Droplet_conc")

Example case: *Kinetics, nanodrop replacement for proteins.*

Ideal for bio-tech, life science and university labs.

### Protein Concentration Determination

Some proteins can be [quantified by absorption spectroscopy](https://knowledge.cphnano.com/en/pages/protein-concentrations-and-refractometry) as aromatic amino acids absorb UV light at 280 nm. Depending on the amount of protein it will absorb more or less light at 280 nm and have a higher or lower extinction coefficient. The extinction coefficient can be calculated theoretically, but these values are often off by 10 % or more. With label-free spectroscopy the extinction coefficient has been determined with high precision. The protein refractive index is a quick way of obtaining the protein concentration without the need of making a standard curve. As an additional advantage, this method also allows for direct quantification of your protein without prior dilution.

#### Application example: 3mL BSA triplicate measurements

 

![PYTH_20210625_BSA_conc_3mL_conc](https://cphnano.com/hubfs/PYTH_20210625_BSA_conc_3mL_conc.svg "PYTH_20210625_BSA_conc_3mL_conc")

Example applications: *BSA, Immunoglobulin, Collagen, Lysozyme, Coronin, Dystrophin, Gloverin, Keratin, Myosin, Tubulin, Insulin, concentration of protein in urine.*

Ideal for bio-tech, life science, food science industry, high schools, environmental research, university and research laboratories.

\*Khatri S. et al. (2021). [White paper: Protein quantification using spectrophotometer, NanoCuvette™ One and SpectroWorks™ compared to micro-volume system.](https://knowledge.cphnano.com/en/pages/protein-quantification-using-spectrophotometer-nanocuvette-one-and-spectroworks-compared-to-micro-volume-system) Copenhagen Nanosystems ApS, Copenhagen (Denmark). Technical University of Denmark, Department of Chemistry, Kemitorvet 206, Kongens Lyngby (Denmark).

## Monitoring Label-Free Enzymatic Reactions

Direct [label-free detection of enzymatic activity](https://knowledge.cphnano.com/en/pages/product-brief) with a substrate can be monitored with the change in the intrinsic property refractive index of the solution as product is formed over time. The NanoCuvette™ One has a built-in optical filter allowing it to measure refractive index changes due to enzymatic reaction kinetics in real time using an UV-Vis spectrophotometer.

![ILLU_20190113_Web_gallery_Application_Notes_Enzymes](https://cphnano.com/hubfs/ILLU_20190113_Web_gallery_Application_Notes_Enzymes.png "ILLU_20190113_Web_gallery_Application_Notes_Enzymes")

#### Superior Technology with no Up-front Cost or Training for Your Lab

## Key Features

[![BLEN_20210813_nanocuvette_animations_one_ref_b](https://cphnano.com/hubfs/BLEN_20210813_nanocuvette_animations_one_ref_b.gif "BLEN_20210813_nanocuvette_animations_one_ref_b")](https://cphnano.com/spectrophotometers/)

![EXTF_20210413_Font_Awesome_box_check_Red](https://cphnano.com/hubfs/EXTF_20210413_Font_Awesome_box_check_Red.svg "EXTF_20210413_Font_Awesome_box_check_Red")

##### SUPPORTS YOUR HARDWARE

NanoCuvette™ One likely works with your existing UV-Vis spectrophotometer. Read more[ here](https://knowledge.cphnano.com/en/knowledge/which-spectrophotometers-are-supported-with-nanocuvette-one).

![EXTF_20210413_Font_Awesome_users_Red](https://cphnano.com/hubfs/EXTF_20210413_Font_Awesome_users_Red.svg "EXTF_20210413_Font_Awesome_users_Red")

##### DESIGNED FOR HUMANS

Easy to use with no special training required. [Online support included](https://knowledge.cphnano.com/en/pages/support-for-nanocuvette-one).

![EXTF_20210413_Font_Awesome_chart_line_Red](https://cphnano.com/hubfs/EXTF_20210413_Font_Awesome_chart_line_Red.svg "EXTF_20210413_Font_Awesome_chart_line_Red")

##### ACCURATE AND INSTANT RESULTS

Results are calculated based on spectral analysis. Our engine in [SpectroWorks™](https://knowledge.cphnano.com/en/pages/what-is-spectroworks) is fast and makes it a breeze to extract results. 

![EXTF_20210413_Font_Awesome_id_card_red](https://cphnano.com/hubfs/EXTF_20210413_Font_Awesome_id_card_red.svg "EXTF_20210413_Font_Awesome_id_card_red")

##### UNIQUE TRACEBILITY

First in the industry, each cuvette is marked for traceability and access to online calibration data.

![EXTF_20220930_Font_Awesome_recycle-solid](https://cphnano.com/hs-fs/hubfs/EXTF_20220930_Font_Awesome_recycle-solid.png?width=68&height=60&name=EXTF_20220930_Font_Awesome_recycle-solid.png "EXTF_20220930_Font_Awesome_recycle-solid")

##### SEMI-REUSABLE CUVETTES

The NanoCuvettes™ can be re-used up to a certain amount [depending on application and maintenance](https://knowledge.cphnano.com/en/pages/nanoucuvette-one-cleaning-manual).

![EXTF_20210413_Font_Awesome_vial_red](https://cphnano.com/hubfs/EXTF_20210413_Font_Awesome_vial_red.svg "EXTF_20210413_Font_Awesome_vial_red")

##### LESS SAMPLE NEEDED FOR ANALYSIS

 Measurements at different volumes (0.5 μL - 3 mL) and concentrations (0.02 - 300 mg/mL)

![PHOT_20210505_vwr_webinar_photoshoot_02_square_resized_1600x1600](https://cphnano.com/hs-fs/hubfs/PHOT_20210505_vwr_webinar_photoshoot_02_square_resized_1600x1600.jpg?width=1600&height=1600&name=PHOT_20210505_vwr_webinar_photoshoot_02_square_resized_1600x1600.jpg "PHOT_20210505_vwr_webinar_photoshoot_02_square_resized_1600x1600")

#### Your Expert in UV-Vis Technology

## Need help getting started? We have got you covered.

[Book Online meeting](https://nanocuvette.cphnano.com/meetings/emiho)

## Ready to try NanoCuvette™ One?

#### Get upgraded from €2 per measurement all included\*

[Book Online meeting](https://nanocuvette.cphnano.com/meetings/emiho)

*\*Purchasement of NanoCuvette™ One at reseller includes life-time access, data storage and quarterly upgrades to SpectroWorks™, free support and  individual calibration for best performance. Price per measurement may vary depending on sample and use.*

# Want to learn more?

### Contact us for more information on your specific instrument.

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Next-Generation Spectroscopy. We empower forward-thinking organisations by delivering innovative and affordable lab and on-site solutions. We make liquid analysis accessible and easy to use.

Winner of *KPMG Global Tech Innovator Award 2022* in Denmark.

Copenhagen Nanosystems A/S  
(cphnano A/S)   
Agern Alle 3,   
DK-2970 Hørsholm,  
Denmark  
CVR/VAT: DK-37293903

Bank Account no.: 3409 11794742  
IBAN: DK1430000011794742  
SWIFT: DABADKKK

#### Shop and Learn

- [NanoCuvette™ One](https://cphnano.com/en/nanocuvette-one)
- [NanoCuvette™ S](https://cphnano.com/nanocuvette-s)
- [SpectroLink™](https://cphnano.com/en/spectrolink)
- [SpectroWorks™](https://cphnano.com/spectroworks)

#### Resources

- [UV-Vis Database](https://knowledge.cphnano.com/en/pages/uv-vis-spectrophotometers)
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#### About cphnano

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