# NanoCuvette™ One

## With NanoCuvette™ One your UV-VIS instrument can now measure 2 mL / 0.5 µL samples via refractive index for QC and kinetics. Read more about the label-free analysis method here.

Absorbance spectroscopy measures how much light a chemical substance absorbs by measuring the intensity of light as a beam of light passes through a liquid sample solution. It is widely used for quantitative analysis in various areas, e.g., chemistry, physics, biology, biochemistry, material and chemical engineering, clinical applications, and industrial applications. This technique requires a sample that absorbs light, but many chemical and biological substances are non-absorbing and other methods must be used.

#### Absorption spectroscopy

The Beer–Lambert law relates the attenuation of light to the properties of the material through which the light is travelling:

where A is the absorbance, ε is molar extinction coefficient, c is the concentration, l is the path length and A0 is the background absorbance.

#### Label-free spectroscopy

When light hits a photonic crystal, the resonance wavelength is related to the refractive index or concentration close to the surface:

where λ is the resonance wavelength, β is a coefficient, α is the specific refractive increment, n is the sample refractive index, c is the concentration, ns is the solvent refractive index and λ0 is the reference resonance wavelength.

### Introducing NanoCuvette™ One

The NanoCuvette™ One is a cuvette that can be used for measuring both refractive index and absorbance using a spectrophotometer. Now you can reliably measure concentrations and kinetics label-free in a standard spectrophotometer without expensive calibration standards and use the SpectroWorks™ software to automatically analyze the results in seconds. Labwork has never easier.
Traditionally, spectrophotometers have been used to measure color intensity by absorbance or fluorescence at a fixed wavelength. With NanoCuvette™ One, a new type of optical filter is inserted into the light beam and the spectrophotometer now measures a wavelength color shift at fixed intensity that is proportional to the analyte concentration or sample change in the cuvette.

## Proteins // Enzymes // Buffers // Foods // Glycerol // Body fluids // Carbohydrates

All-round solution

• Excellent for QC, kinetics, and label-free concentrations of a wide range of samples.

• All major UV-VIS spectrophotometer brands supported seamlessly out of the box.

Affordable analysis

• Based on semi-disposable PMMA cuvette, reusable for low-cost measurements.

Designed for people

• Results independent of cleaning, pipetting errors, and transcription mistakes.

## Overview

The optical filter inside the cuvette consists of nano-scale photonic crystals with a light guiding measurement principle similar to Surface Plasmon Resonances (SPR). Both the absorbance spectrum and the refractive index of the sample can be analyzed.

Powerful performance

• Combined surface refractive index range 1.0 – 1.6 nD and absorbance at 300-1100 nm.

0.5 µL / 2 mL samples

• Both 0.5 µL and 2 mL measurements with one product in UV-VIS instruments.

Unique ID

• Each cuvette is marked for traceability and access to online calibration data.