With NanoCuvette™ One your UV-VIS instrument can now measure 2 mL / 0.5 µL samples via refractive index for QC and kinetics. Read more about the label-free analysis method here.
Absorbance spectroscopy measures how much light a chemical substance absorbs by measuring the intensity of light as a beam of light passes through a liquid sample solution. It is widely used for quantitative analysis in various areas, e.g., chemistry, physics, biology, biochemistry, material and chemical engineering, clinical applications, and industrial applications. This technique requires a sample that absorbs light, but many chemical and biological substances are non-absorbing and other methods must be used.
The Beer–Lambert law relates the attenuation of light to the properties of the material through which the light is travelling:
where A is the absorbance, ε is molar extinction coefficient, c is the concentration, l is the path length and A0 is the background absorbance.
Label-free spectroscopy
When light hits a photonic crystal, the resonance wavelength is related to the refractive index or concentration close to the surface:
where λ is the resonance wavelength, β is a coefficient, α is the specific refractive increment, n is the sample refractive index, c is the concentration, ns is the solvent refractive index and λ0 is the reference resonance wavelength.
Introducing NanoCuvette™ One
The NanoCuvette™ One is a cuvette that can be used for measuring both refractive index and absorbance using a spectrophotometer. Now you can reliably measure concentrations and kinetics label-free in a standard spectrophotometer without expensive calibration standards and use the SpectroWorks™ software to automatically analyze the results in seconds. Labwork has never easier.
Traditionally, spectrophotometers have been used to measure color intensity by absorbance or fluorescence at a fixed wavelength. With NanoCuvette™ One, a new type of optical filter is inserted into the light beam and the spectrophotometer now measures a wavelength color shift at fixed intensity that is proportional to the analyte concentration or sample change in the cuvette.
Excellent for QC, kinetics, and label-free concentrations of a wide range of samples.
Fits your instrument
All major UV-VIS spectrophotometer brands supported seamlessly out of the box.
Affordable analysis
Based on semi-disposable PMMA cuvette, reusable for low-cost measurements.
Designed for people
Results independent of cleaning, pipetting errors, and transcription mistakes.
Overview
The optical filter inside the cuvette consists of nano-scale photonic crystals with a light
guiding measurement principle similar to Surface Plasmon Resonances (SPR). Both the absorbance spectrum and the refractive index of the sample can be analyzed.
Powerful performance
Combined surface refractive
index range 1.0 – 1.6 nD and
absorbance at 300-1100 nm.
0.5 µL / 2 mL samples
Both 0.5 µL and 2 mL measurements with one product
in UV-VIS instruments.
Unique ID
Each cuvette is marked for traceability and access to online calibration data.
Made in Denmark
Danish product manufactured under the highest quality standards.
Using a round glass slide, the sample volume can be reduced.
Proteins can be expensive and cost up to 20.000 €/mg. Therefore, we have developed a method that only uses a minimum of protein to determine 2-400 mg/mL concentrations.
You can now measure low volume 0.5 µL protein samples at low cost in seconds using a conventional spectrophotometer by dispensing a droplet and flattening it on the optical filter of NanoCuvette™ One. This method enables quick undiluted measurements of relevant static
parameters with the lowerst sample volume of any solution on the market today.